Calcium ion cycling in rat liver mitochondria
نویسندگان
چکیده
منابع مشابه
The regulation of extramitochondrial free calcium ion concentration by rat liver mitochondria.
The mechanism whereby rat liver mitochondria regulate the extramitochondrial concentration of free Ca(2+) was investigated. At 30 degrees C and pH7.0, mitochondria can maintain a steady-state pCa(2+) (0) (the negative logarithm of the free extramitochondrial Ca(2+) concentration) of 6.1 (0.8mum). This represents a true steady state, as slight displacements in pCa(2+) (0) away from 6.1 result in...
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The impermeability of the mitochondrial inner membrane to the chelator ethanedioxybis(ethylamine)tetra-acetic acid permits discrimination between Ca(2+) which has been transported to the internal (matrix) phase and Ca(2+) which binds to the external surfaces of the mitochondrion. With this technique, it is shown that ;energy-independent high-affinity' binding is a measure of carrier-mediated ac...
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BACKGROUND AND PURPOSE The impairment of mitochondrial respiratory function is an important problem in anoxia-reoxygenation injury in animal cells. This study investigated the role of mitochondrial calcium ions (Ca2+) in a model of anoxic incubation in isolated rat liver mitochondria. METHODS Mitochondria isolated from Wistar rat liver were subjected to anoxic incubation for 5 to 60 minutes. ...
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Initial velocities of energy-dependent Ca++ uptake were measured in rat liver mitochondria by stopped flow techniques and dual wave length spectroscopy in the presence of the Ca++ indicator murexide. The initial rate of energy-dependent Ca++ uptake by mitochondria was 0.1 to 0.3 nmole per s per mg of protein at 5 to 15 pM Ca++, and increased to 8.2 nmoles of Ca++ per s per mg at 200 PM Ca++. Wh...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1978
ISSN: 0306-3283
DOI: 10.1042/bj1740613